Carter A. Mitchell

National Cancer Institute – Frederick

Molecular Targets Laboratory

Cell: (904) 252-1638

1050 Boyles St
Frederick, MD 21702


National Cancer Institute – Frederick
Post Doctoral Research Fellow

Jan. 2015 – Present
Advisor: Barry O’Keefe, Ph.D.

Research area: Isolation of bioactive proteins from marine aqueous extracts.

University of Oklahoma, Norman, OK
Post Doctoral Research Associate

August 2013 – Jan. 2015
Advisor: Robert H. Cichewicz, Ph.D.

Research area: Optimization of the Mammalian Microbiome pipeline for isolation and identification of novel anti-cancer and anti-microbials. Included initial collection, fermentation in three different media types, and activity guided fractionation for structure solution. At the height of operation we were screening ~200 bacterial isolates a week against MRSA, Candida, and Pancreatic cancer cell-lines.

University at Buffalo, Buffalo, NY
Ph.D., Structural Biology                

August 2013
Advisor: Andrew M. Gulick, Ph.D.

Research area: Structural and functional characterization of domain choreography in Non-ribosomal Peptide Synthetases and identification of a putative virulence factor from a cryptic biosynthetic operon.


Texas State University, San Marcos, TX
B.S., Biochemistry, Summa Cum Laude

August 2005
Advisor: Rachell E. Booth, Ph.D.

Research area: Characterization of the assemblage and stoichiometry of the amiloride-sensitive epithelial sodium ion channel (ENaC) subunits.



Postdoctoral Research Fellow
Jan. 2015 – Present
PI: Dr. Barry O’Keefe, National Cancer Institute – Frederick

Postdoctoral Research Associate
Aug. 2013 – Jan. 2015
PI: Dr. Robert H. Cichewicz, University of Oklahoma

Stafford Fellow
May 2008-Aug. 2013
PI: Dr. Andrew M. Gulick, HWI/UB

Research Assistant/Lab Manager
Aug. 2005-June 2007
PI: Dr. Arturo Delozanne, University of Texas – Austin

Research Assistant
Aug. 2001-Aug. 2005
PI: Dr. Rachell Booth, Texas State University – San Marcos

Instructional Assistant
Aug. 2003-Dec. 2004
Under Dr. Debra Feakes, Texas State University – San Marcos



Mitchell, C.A., Shi, C., Aldrich, C., Gulick, A.M. Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains. (2012) Biochemistry. 51 (15), p 3252-3263.

Mitchell, C.A., Tucker, A., Escalante-Semerena, J.C., Gulick, A.M. The Structure of S. lividans Acetoacetyl-CoA Synthetase Shows a Novel Interaction Between the C-terminal Extension and the N-terminal Domain. (2014) Proteins. 83 (3), p 575-581.

Stamps, B.W., Du, L., Mitchell, C.A., Cichewicz, R.H., Stevenson, B.S. Draft Genomes of Two Sordariomycete Fungi That Produce Novel Secondary Metabolites. (2015) Genome Announcements. 3 (2).

Wilson, B.A.P., Alum, M.S., Guszcyznski, T., Jakob, M., Shanoy, S.R., Mitchell, C.A., Goncharova, E.I., Evans, J.R., Wipf, P., Liu, G., Ashwell, J.D., O’Keefe, B.R. Discovery and Characterization of a Biologically Active Non-ATP-Competitive p38 MAP Kinase Inhibitor. (2015) Journal of Biomolecular Screening. Epub 11/04/2015.

Motley, J.L., Stamps, B.W., Mitchell, C.A., Thompson, A.T., Cross, J., You, J., Powell, D.R., Stevenson, B.S, Cichewicz, R.H. Opportunistic Sampling of Roadkill as an Entry Point to Accessing Natural Products Assembled by Bacteria Associated with Nonanthropoidal Mammalian Microbiomes (2016) Journal of Natural Products. Epub 11/21/2016.

Mitchell, C.A., Ramessar, K. O’Keefe, B.R. Antiviral Lectin: Selective Inhibition of Viral Entry (2017) Antiviral Research. 142 37-54.



Polymastin: A Marine Sponge Derived Protein with Potent Anticancer Activity, Poster
FYI Colloquium, Shady Groove, MD – March 2017

Vagocin: An Anti-HIV Protein Isolated from the Marine Sponge Vagocia sp., Poster
FYI Colloquium, Shady Groove, MD – March 2016

Vagocin: An Anti-HIV Protein Isolated from the Marine Sponge Vagocia sp., Poster
Spring Research Festival, Frederick, MD – May 2016

Insights into NRPS Domain Choreography: Structure and Molecular Dynamics, Poster
Enzyme Mechanisms Conference, Coronado, CA – Jan. 2013

Insights into the Domain Choreography of NRPSs, Presentation
Buffalo/Hamilton/Toronto Crystallography Conference, Hamilton, ON – Nov. 2011

Structural Investigation of a Novel NRPS Cluster from Pseudomonas aeruginosa, Poster
Enzyme Mechanism Conference, St. Petersburg, FL – Jan. 2011
Witebsky Conference on Microbial Pathogenesis, Buffalo, NY – May 2011

Identification of the Subunit Stoichiometry of the Amiloride-Sensitive Epithelial Sodium ion Channel, Poster
American Society of Biochemistry and Molecular Biology Experimental Biology Conference, San Diego, CA  – April 2005



NCI Innovation Award: Anti-Cancer Protein: A Novel Paradigm to Fight the Ancient Battle
FY 2017

NCI Cancer Research Training Award
Jan. 2015 – Present

Stafford Fellow Research Training Award
Jan. 2008 – Aug. 2013


Conferences and Workshops

CCR FYI Colloquium, Shady Grove, MD
March 2016

PEAKS Training Workshop, Bethesda, MD
Feb. 2016

Enzyme Mechanisms Conference, Coronado, CA
Jan. 2013

Biodynamics@Buffalo, Buffalo, NY
July 2012

BioSAXS Workshop, CHESS, Ithaca, NY
April 2011

Enzyme Mechanism Conference, St. Petersburg, FL
Jan. 2011

Witebsky Conference on Microbial Pathogenesis, Buffalo, NY
May 2008-12

Buffalo/Hamilton/Toronto Crystallography Conference, Hamilton, ON
Nov. 2008-11

Experimental Biology – ASBMB, San Diego, CA
April 2005



Mentor – Werner H. Kerstin Intern Program – NCI – Frederick
May, 2016-Present

Proctor and Judge – First Year Research Experience – OU
April, 2014

Proctor and Judge – Structural Biology Section New York Science Olympiad
March, 2011-12

Head Organizer of the Graduate Student Distinguished Lecturer for HWI
2010 & 2013


Organizations and other Activities

American Society of Pharmacognancy member

American Crystallography Association member

American Chemistry Society member

Broadening Excellence in Experimental Research, co-founder and pres

Structural Biology GSA, Secretary

IGPBS Graduate Student Recruitment

Golden Key National Honors Society

Phi Sigma Pi National Honors Fraternity
Service Committee Chair and Parliamentarian

Biochemistry and Pre-Pharmacy Undergraduate Mentor
Aug. 2002-04